Twenty-several Lythraceae variety had 383 long repeats off four brands

Of one’s twenty-two Lythraceae variety, L. subcostata is actually new quickest (152,049 bp), and L. villosa try this new longest (160,769 bp). Apart from L. villosa, new lengths of the cp genomes out of Lagerstroemia species ranged between 152,049 bp and 152,519 bp, while the cp genomes of one’s most other genera from Lythraceae varied from 155,555 bp to help you 159,380 bp (Table step one). Generally speaking, the latest cp genomes of thirteen Lagerstroemia varieties was somewhat smaller than those of other Lythraceae. New stretched duration of the cp genome regarding L. villosa resembled the ones from the fresh six freshly sequenced types of Lythraceae more than it resembled the Lagerstroemia types. The fresh lengths of one’s intergenic places (IGS) varied out-of forty two,031 bp so you can 46,156 bp one of several 13 Lagerstroemia varieties and forty five,923 bp so you can 51,357 bp one of the remaining species of Lythraceae, that was from inside the accord towards lengths of your own done cp genomes (Table cuatro). As in almost every other angiosperm flowers, differences in IGS size contributed greatly to your variation when you look at the genome size. Brand new percentage of GC articles from the chloroplast genomes of twenty two varieties are –%, with an average of %. The typical GC content from Lagerstroemia varieties is actually %, which was more than that of another genera (%).

Brand new genomic construction, such as the count and you may succession off family genes, try extremely saved one of many twenty-two Lythraceae species. not, there are structural changes in the latest IRA and you can IRB boundaries (Fig. 3). As the IR part is much more stored as compared to most other countries, the brand new improvement and you may contraction off IR boundaries played a primary part when you look at the genome proportions [34,35,36].

Research from ong twenty-two kinds. Length regarding shape is not to help you measure. LSC, Large solitary-copy; SSC, Small solitary -copy; IR, upside-down recite

This new items of the Irs varied out of twenty four,421 bp (T. natans) to help you twenty six,907 bp (L. villosa). From inside the IRA-LSC limitations of the twenty two variety, brand new limitations off 18 types decrease in the rps19 programming gene and you will caused an rps19 pseudogene from the IRB region. The latest IRA-LSC border of L. villosa try on the remaining side of the rps19 coding gene plus the IRA-LSC boundaries away from D. grandiflora, W. fruticosa and you will H. myrtifolia was in fact found on the right of rps19 programming gene. The length anywhere between rps19 therefore the IRA-LSC edge varied away from 3 bp to 279 bp.

Except for the latest fourteen Lagerstroemia types and you will W. fruticosa, new IRA-SSC boundary is actually stuck regarding the ndhF encryption gene and had a duration of 7 bp (W. fruticosa) so you’re able to 158 bp (L. guilinensis) regarding the IRA region. With the almost every other seven Lythraceae varieties, ndhF was found on the right-side https://www.datingranking.net/tr/caribbeancupid-inceleme of your own IRA-SSC well away off twenty-eight bp in order to 55 bp of the new boundary. For everybody species, the brand new SSC-IRB border is found in the ycf1 gene that have a length regarding 1062 bp so you can 2252 bp about IRB part, leading to a beneficial ycf1 pseudogene throughout the IRA region that have a matching duration. Brand new trnH-GUG noncoding gene are located on the right side of the IRB-LSC edge anywhere between 69 bp so you’re able to 75 bp from the a great point of 0 to 33 bp regarding the IRB-LSC edge.

Long repeat structure data

To get types had merely give and you will palindromic repeats, and simply T. natans got all kinds of repeats. L. indica had the biggest number of repeats, in addition to twenty two send and you can half dozen palindromic repeats. W. fruticosa, P. granatum, L. salicaria and P. granatum got simply seven a lot of time repeats. Total, H. myrtifolia as well as the 14 Lagerstroemia types got far more long repeats than just D. grandiflora, T. natans, L. salicaria, L. inermis, P. granatum, W. fruticosa and you can Roentgen. rotundifolia (Fig. 4a, Additional document 4: Desk S4). The newest copy size varied off 30 bp so you can 81 bp. Recite sequences off 31, 30 and you can 41 accounted for all of the complete size (Fig. 4b).